Mutations in the gyrA, parC, and parE genes associated with fluoroquinolone resistance in clinical isolates of Mycoplasma hominis.

نویسندگان

  • C M Bebear
  • J Renaudin
  • A Charron
  • H Renaudin
  • B de Barbeyrac
  • T Schaeverbeke
  • C Bebear
چکیده

Five clinical isolates of Mycoplasma hominis from three different patients were examined for resistance to fluoroquinolones; some of these isolates were probably identical. All five isolates harbored amino acid substitutions in the quinolone resistance-determining regions of both DNA gyrase (GyrA) and topoisomerase IV (ParC or ParE). Furthermore, the novobiocin MIC for three isolates showed a significant increase. This is the first characterization of fluoroquinolone-resistant clinical mycoplasma isolates from humans.

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Molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis clinical isolates

To evaluate the molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis (MH) clinical strains isolated from urogenital specimens. 15 MH clinical isolates with different phenotypes of resistance to fluoroquinolones antibiotics were screened for mutations in the quinolone resistance-determining regions (QRDRs) of DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE) in ...

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Alterations in topoisomerase IV and DNA gyrase in quinolone-resistant mutants of Mycoplasma hominis obtained in vitro.

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تعیین الگوی مقاومت آنتی‌بیوتیکی و بررسی موتاسیون در ژن‌های gyrA و parC در سویه‌های اشریشیاکلی جدا شده از بیماران مبتلا به عفونت ادراری

Introduction & Objective: Fluoroquinolones are essential antimicrobial agents used to treat UTIs. Clinical experiences have shown a high rate of antibiotic resistance among uropathogens. These resistance are usually the consequence of mutations involving genes encoding gyrA and parC. The aim of this study was to determine antimicrobial resistance pattern and the presence of mutations in regions...

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عنوان ژورنال:
  • Antimicrobial agents and chemotherapy

دوره 43 4  شماره 

صفحات  -

تاریخ انتشار 1999